FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1976-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1976-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766264
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT13360.1743524425002349No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA7980.10414165352933193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.0123.210481
GTGATCG1700.080.966389
GCGAAAG3150.078.213542
TACGGGG1350.075.1217967
ATATACG1350.075.116884
TATACGG1550.065.407295
AGACGGT702.3549E-762.062076
CGTATGC502.0183425E-457.951098
GTATATG1154.0017767E-1156.720941
ATACGGG1950.051.9836166
CGAAAGC4950.048.2957343
TTAGAGT2850.048.2894254
GAGTGAT3100.046.7347537
AGCGGTA4650.045.1621745
TAGCGGT4700.044.69344
GTATGCG657.363669E-444.580689
ATAGCGG5050.041.6012763
GCAGGTA2800.041.4071662
TATAGCG5150.040.804152
ACTGATC3250.035.6622128
Produced by FastQC (version 0.11.5)