FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1975_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCATTAAC23.125No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA23.125No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG23.125No Hit
GAATGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGA23.125No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC11.5625No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT11.5625No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA11.5625No Hit
GTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGCCCTATCAATA11.5625No Hit
GGCTCACATAACCCCGGGGGGGCAGGGGGAGGCCACGGCAGATCAGGAAG11.5625No Hit
GGCCAAGAGCATGGAATATGATGCCGTTGCAACTACACATTCATGGATTC11.5625No Hit
CTATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAA11.5625No Hit
TATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAG11.5625No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG11.5625No Hit
GGGACCCACAGCACTACACCCAGGCCCTCGGCCACACACAGAGTCAGAAA11.5625No Hit
CGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTC11.5625No Hit
TCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAA11.5625No Hit
GCTAAGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT11.5625No Hit
GTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTCCG11.5625No Hit
NNNNNNNANTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11.5625No Hit
CTTCCATTTCATTGACGAACGAGGCGAATCAATAATTGTGGAATCTGGTG11.5625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.5625No Hit
GGCTCCAGGCGGGCCGAGGGAGAGACCTGACAAAGCATCTCAAGACGGCC11.5625No Hit
AGGGAAGACTGTGTAACCCTATGAACCCGTTTGTCAGTCATAAGGAAGTT11.5625No Hit
GCCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGACCGACCATTA11.5625No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG11.5625No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG11.5625No Hit
TGTCAATGGTCTGCAGGGAAGAATTGATTATTATTGGTCGGTACTAAAAC11.5625No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA11.5625No Hit
AGTTAGGTGGGAGACTTTGGTCGGCAAGCCTACGCATGGTCCCTGTGATT11.5625No Hit
GTAAAGAGGAATAACAATTCCAGTGCTCACAAGCAGAAATGATGGCTAAT11.5625No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG11.5625No Hit
CCTAAACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGAT11.5625No Hit
GTGTAGTGCAATGTCAGACTGAAAAAGGTGGCTTAAACAGTACATTGCCA11.5625No Hit
GGGTTGAATTTTAGGCCAAGTTCCCTCTTAACTTGTCAGAACCTGGACCC11.5625No Hit
AACAAGCCTATCTAAAATATCTCACCATCCCAATACTCACTGTCAAAGAA11.5625No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA11.5625No Hit
GCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTG11.5625No Hit
GTGGATATGAATTTGAGAGGGAAGGGTACTCGATGGTTGGGATAGATCAT11.5625No Hit
GTATAAATTCGGCCCTCCATCTGAAACCAACAGTCCTGCCTTTGGGCGGG11.5625No Hit
GAGCAATGATGGATCAGGTGCGAGAAAGCAGAAATCCTGGGAATGCTGAA11.5625No Hit
CATCATAAATGATGCTAGCAGTCGCATTTCTATCATCCCCAGTGACACAA11.5625No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA11.5625No Hit
CCCCTGCAGCTGGGGAGCCCCCGGGAACTCTGCTCTCCAGAAGAGAATGG11.5625No Hit
TATTCATCCACCCCCATTTTGCTAACTCTCACTCCTCTTAGTGACATTTC11.5625No Hit
CTCATTGGCTGTTAGACCGTTCGATCTGAATACCTCTATGGTGTTGGCCA11.5625No Hit
CATGAGAACTGGGGAGTCAGTTCTCTGACAGGGTACTGTGTGTGTATGTG11.5625No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA11.5625No Hit
AGTAGAAACAAGGCATTCGCAGTTCGAGCATCCGAACCTGCTTTCGCTCC11.5625No Hit
AAAAGAAGTCTTACATAAATCGGACAGGAACATTTGAGTTCACAAGCTTT11.5625No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC11.5625No Hit
ACATTTGAGTTCACAAGCTTTTTCTACCGCTATGGGTTTGTAGCCAACTT11.5625No Hit
GTTCCAAATGCATATTTACTGATATTGTGGAATGGCAATGTACTGTTTAA11.5625No Hit
GTAAGTCACATTCCAGGTTGTGTCTGGGAAGATTTGGATTCTTTGGTAGG11.5625No Hit
CCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCA11.5625No Hit
GCACTATGGGGGTGATTTTAGAATGCGTGAGTTTTGCTCCTTTCGTATGC11.5625No Hit
ATATAAATGAGCACGGACTGAAGCGAGCTCCTGGCCAGAGAAACCCTATG11.5625No Hit
TTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACAA11.5625No Hit
GTGCTTGCCTCTGCTCGAGCAGGAGCTGCCCCAGGGAAGGGACGGACGGG11.5625No Hit
GAACTCAGGACACTTTTTAGTTCCGCTAGTTCCTACCAAAGAATCCAAAT11.5625No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTATGGCATGTCC11.5625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA50.0145.07
GTTTTAG50.0145.01
TTTTAGT50.0145.02
ACCGACC50.0145.09
AGTACCG50.0145.06
TAGTACC50.0145.05
TACCGAC50.0145.08
GCTCGGT50.0145.0145
TTTAGTA50.0145.03
TTAGTAC50.0145.04
Produced by FastQC (version 0.11.5)