FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1975_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819409
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG2200.046.1005449
AAGTACG657.3639164E-444.5807467
CATAGCG1209.620835E-636.217433
TTAACGC2601.1277734E-1030.6455213
CGAAAGC5600.028.4652442
TAGCGTG1804.7383182E-628.1639525
AGTGATC4200.027.5976077
TCGTGTC1100.0097072626.3431689
TCATAGT5350.025.724533
TCTATAC5350.025.724533
GCATTAT1450.001227874924.9943311
ATAGCGT2051.29952405E-524.7323444
TACATAG3551.5643309E-1024.4880168
TATGCTG5050.024.3795455
TATACAG4850.023.8916595
AGGATCG1550.001819628823.3618055
ATTAACG3554.150934E-922.4514622
ATATACC4205.2750693E-1122.4189454
GCGTGTT1952.6279554E-422.290377
AAACGTT1952.6323035E-422.2849256
Produced by FastQC (version 0.11.5)