FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1975_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977943
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCA1203.6379788E-948.3071447
TATTACG1201.9833897E-742.2644234
AGCGAAA5600.041.416721
GCGAAAG6100.038.0121772
TATCGCA800.002042017636.230367
CGAAAGC7250.032.978743
CGCTATA1306.503418E-427.8695072
ATTACGT1907.1939776E-626.6919545
GAGCGCG1958.791121E-626.011549
ACCGATG1500.001498972424.153578
ACCGTGT2502.2898912E-623.1874288
GTATACT2252.6646927E-522.5410254
CATTACT5001.8189894E-1221.7359894
CTGTGCG4701.0913936E-1121.5840429
ATTACTC5151.8189894E-1221.1018225
GCACCGT2755.237298E-621.0784026
GTACTCA6300.020.7009414
TATAGTA2806.1268E-620.6998835
ATAGACC2155.078033E-420.2195243
ACGTCAG2556.986328E-519.8911748
Produced by FastQC (version 0.11.5)