FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1975_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714355
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10670.1493655115453801No Hit
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA8340.11674867537848829No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT203.8786946E-4108.657584
GTGATCG2800.067.278359
GAGTGAT4300.043.8091627
AGCGAAA2000.043.4843561
TAGAGTG6250.037.0832565
AGTGATC5150.036.5785268
TTAGAGT5750.036.534144
GTAATAC1406.650207E-736.2191963
GTATTAG1905.4405973E-934.3297541
GCGAAAG2603.6379788E-1233.442472
GTCCTAT6600.032.9426961
GTATACT2455.638867E-1132.5233574
CCTTACG900.003637677832.2038652
AGAGTGA7450.031.1101156
ACCGTGC950.00474121830.5067838
TTTAGAG8350.030.3633963
TAGTATA2252.8303475E-828.9834772
CGTAAGG1050.007728460227.603312
GCCGACC1100.00964707726.37634145
GTATAGG1100.0096867326.3541551
Produced by FastQC (version 0.11.5)