FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1975-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073638
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15870.147815185379057No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15300.14250613335220996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTC1001.3976044E-436.2231068
TAAGACG1102.4455643E-432.9331674
AGTCCGC2755.456968E-1231.658659145
GCGATTA1153.1492117E-431.54395145
AAACGCG950.004711183730.547827145
CCTTCGC1801.5280594E-424.1487399
GTTGACA11200.023.9420551
TACTGAA16950.023.9350327
GTATATA7050.023.6437551
GTCTAAC1851.835371E-423.5048371
CGGTTCA1550.001816399723.3697459
CTACTGA18100.023.2137266
GTAGCAT19400.023.1615541
AGCGAAA4800.022.647891
ACATGGT12550.022.5152665
TATACTG4557.2759576E-1222.293225
GCGAAAG4701.0913936E-1121.5857622
GCATGTT16650.020.8873424
TATACTA2806.125245E-620.7008485
CAGGTCG6300.020.6989177
Produced by FastQC (version 0.11.5)