FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1975-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606472
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1152.5083864E-950.372519
TATACTG3500.035.1655
GTAATAT2900.032.4867021
CGCTTAC955.628189E-430.513821
TAGAGTG2901.2732926E-1129.9580125
AATAGGG2156.153077E-726.9590232
AGGTCGG2051.3015724E-524.725538
TTAGAGT3551.5643309E-1024.4767844
ATACTGT5750.023.9249676
GCGAAAG3753.092282E-1023.1905061
CTTAGAC1600.002186680222.6338863
AGCGAAA3655.700713E-921.8403721
GGGATAC3457.433118E-821.030226145
CCTATAC1750.00367807520.6938363
GAATAGG3208.492643E-720.382281
CGAAAGC4702.382876E-1020.0400182
GAGTGAT2908.329111E-619.9753077
TAATATA4402.2755557E-919.7597352
AAACGCT1850.005022826619.609264145
CCTAATA1850.005063825319.581722
Produced by FastQC (version 0.11.5)