FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1975-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856806
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12740.1486917691986284No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11320.13211858927225067No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9570.11169389570101049No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9210.10749224445206966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1850.050.9265331
TTACGTG2150.047.1913222
AACCGTA700.001059756341.4031077
CGTGTTG2500.040.5679325
CGAACAC750.001488181638.6406443
ATCGAAT951.026118E-438.174595145
CTACTGA13850.036.0927166
ACGTGTT2900.032.4781274
TACTGAA15450.032.3587387
AGCGAAA3600.032.2099461
TATACTA1902.090419E-730.5022035
GCGAAAG4050.030.4205042
ACCGTAG1204.072202E-430.1897688
CATTCTA10400.029.267692
ACTGAAC18550.028.1228668
GTAGCAT13800.027.3084341
CGTTATA1358.099757E-426.8416231
TAGCATG10400.026.4802912
CGAAAGC4750.025.9299053
GTTATGT6150.025.9250771
Produced by FastQC (version 0.11.5)