FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1975-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800678
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAT450.00965441448.3130721
GTGCGAA1105.296324E-639.50914
GCGAAAG3500.039.335722
AGCGAAA3600.038.247851
CGAAAGC4000.034.4079933
GTACTAG1402.7538703E-531.0584011
TCAGGCG3005.9299055E-1026.5605589
CGGAGTA1701.0375034E-425.5647134
CGTATGG1500.001501487224.1459623
TGTGCGA1851.8408823E-423.493373
GGAACCG2202.24398E-523.0469765
GTTGACA5750.022.6861361
ACTAACG1600.00218563222.6368399
GTATTAG2003.1110924E-421.740881
AGGGTGT5101.8189894E-1221.3039286
GACTTAA4501.3278623E-1020.9356611
TGCGAAG2104.33994E-420.6952465
TATACTG4201.2823875E-920.6952465
AAACGCT2505.940159E-520.31436145
GTCCTAT8350.019.9616891
Produced by FastQC (version 0.11.5)