FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1975-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1975-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859653
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12980.150991155733767No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT10490.1220259802501707No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA10110.11760559202375843No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9920.11539539791055228No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA8690.10108729917769146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1500.072.467061
TTACGTG1800.060.3751372
GTCTACG752.5571404E-548.311371
TACGCCT907.5222604E-540.2407154
CGTGTTG2750.039.5044675
ACGTGTT2850.038.1227844
AATCGCT1051.8610018E-434.500082
GTTATGT5050.034.439791
TCTACGC1102.445715E-432.9318922
GTTGACA6900.031.5074141
GAATCGC1153.170168E-431.5074141
GTAATAT2101.4457328E-831.0573061
AACCGAT1402.759156E-531.0500742
GTAACGG1652.3943794E-630.7435971
AGGCGCG1255.181079E-428.9733167
TACTGAA10750.028.2995177
CTACTGA10350.027.990286
CAATACT1306.5171794E-427.8589574
TACGTGT3950.027.5063113
GTATTAT9200.026.7813011
Produced by FastQC (version 0.11.5)