FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1974-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1974-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020021
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGT950.004749535630.4970914
GTTACGT1306.4951635E-427.87571
TACCGTG1100.00972622426.333237
CAACCCG3451.07320375E-1025.237831145
AGCGAAA3551.546141E-1024.4992081
ACCGTGC2101.5690654E-524.1387928
TTACGTG1550.001814986223.3727362
GCGAAAG4058.112693E-1021.4682922
TATACTG5850.021.0440775
TATACAG5900.020.8657365
GTATAAG3508.766074E-820.7076661
CAGCAAC7000.020.6903957
GCAGTGC8500.020.4640451
TATAACC4904.1836756E-1019.2200533
GTTGACA8050.018.9069981
CGAAAGC5005.475158E-1018.8356533
GTTATGT7000.018.6368981
GTCTATC2751.245275E-418.4486471
GTATAAC3151.6882188E-518.4068131
GTATATC2400.001062004818.1192071
Produced by FastQC (version 0.11.5)