FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1974-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1974-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413467
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG6970.16857451743428134No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC5800.14027721680327573No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA5390.13036106871890624No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG5120.12383092241944338No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA4970.12020306336418625No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC4970.12020306336418625No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA4630.11197991617227009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT650.0111.4861451
TTACGTG903.6379788E-1272.4572142
CCTTCGC651.0979145E-555.7228169
GACGACT400.006077066554.314
CATAGCG450.00966690148.2931067
ACGTGTT1351.9281288E-1048.275564
CGTGTTG1402.746674E-1046.5401575
CCGACTA1652.910383E-1143.886874
TTACCGC700.001058358241.4091451
CGCACTT700.00106341841.369035
GATATAC1209.612901E-636.215443
TACGTGT1854.2091415E-935.2366453
TATACTG1854.2291504E-935.2195785
CCACCGA2103.783498E-1034.5076181
CTACTGA6100.033.2429356
CTTATGA2051.1403245E-831.8143421
CACCGAC2301.0059011E-931.5031382
TACTGAA6950.031.2689177
GTTATGT2601.10958354E-1030.6586911
TACTATA1204.0679998E-430.1905062