FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1974-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1974-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108600
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT68460.6175356305249864No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA54870.4949485837993866No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA29480.2659209814180047No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG27970.2523001984484936No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT27740.25022550965181306No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG27110.24454266642612305No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG24540.22136027421973659No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA23830.21495580010824464No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG22930.20683745264297315No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA22130.199621143784954No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC22000.19844849359552588No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG21350.19258524264838534No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC20290.18302363341151No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG19450.17544650911058993No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT18820.16976366588489988No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC17960.1620061338625293No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG17340.1564134944975645No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG16830.15181309760057732No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT16820.15172289373985207No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT16200.14613025437488725No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA16180.14594984665343677No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA14520.13097600577304708No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT13730.1238499007757532No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA13240.11942991160021649No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG12220.11022911780624212No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11900.10734259426303447No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG11860.1069817788201335No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT11560.10427566299837632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAA20400.063.5752532
GCGCGTT400.006033660454.41458145
ACAAGGC20300.052.0959978
AGCGAAA6050.051.510531
TAGAAAC25400.051.0558243
CAGTATG9700.050.032329
GACAGTA10000.049.980047
AGTAGAA26300.049.3264921
GCAGGTA6950.048.997992
AGCGACG1650.048.3029822
CAAGGCA22100.047.8528869
AAGGTCG6900.047.2401169
GGTACTG7200.046.271555
TTGGACA11000.046.0928234
GCGACGA1751.8189894E-1245.5386963
ACAGTAT10950.044.9823658
GCGAAAG7050.044.1920932
ATCCAAT16150.043.954277
AACAAGG31700.041.8156977
CGAAAGC7450.041.815583
Produced by FastQC (version 0.11.5)