FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1973-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1973-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564148
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA16620.2946035437509306No Hit
GTGTTTAGGTCCTGGATTTTTAAGGAAAAACCCTTCGGTCTGAAGACTTT10130.1795628097591412No Hit
TCTGTAGAGTGGGGATAATAATGGTAAACATTTAATTGGGTTGCTAGGTT8860.15705098662053218No Hit
GCTTTATACTGGGGCATTGTATGTTCGAGAAGCAATGTTCTATAAGAGGA8300.147124513425555No Hit
GTTTAATAAGATGGGGAGACTGGGACAGGAGCAGGTTGAGGAGGGATCAG8270.14659273807582407No Hit
ATTATGACCTGCGTGTACAATTTCTTAATGATAATTGTTGTCCACAAACC7590.13453916348192319No Hit
TCCCAGGACTCTGGGATCACAATCCAAGCTGAAGGCAGACCCTCAACCGA7340.1301077022341655No Hit
GGATGAGGGAGGACACAGGCCTGTGTTTAGGTCCTGGATTTTTAAGGAAA7210.1278033423853315No Hit
CCTTATAAGAGAGTTGGAACTCTTACCTGTGCCAAGGAATACTTCACCAC6840.12124477973865014No Hit
CTCTACCTCTGTCATTACATGCATATTTGCATGACAACAGACCATTTTTC6220.11025475584421109No Hit
CTTAATAGACTGAGCCACCATGGTGTCCTAAAAAATTAATTTTCTAAAAT5790.10263264249806789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG3250.080.249669
AGCGAAA3200.079.2747341
GCGAAAG4000.063.402912
GAGTGAT4450.058.60937
AGTTCCG502.0166808E-457.9580888
GTACGTA400.006056205454.3598181
TAGAGTG8950.049.373325
CGAAAGC5150.049.2449763
TTAGAGT5700.045.7604454
AGAGTGA5700.045.7523276
TAGCGTA657.308678E-444.646523145
GTGGTCA2650.043.741959
TATACTC2500.043.472424
GCAGTTC700.001060474241.3949626
AGTGATC6600.040.6145678
TATATCT1303.7129576E-739.0137144
ATAAGAT4700.038.5325436
CTGCTAA951.0300109E-438.1472431
CAGGGCG951.032706E-438.130329
TCCACCG1752.408342E-937.311737145
Produced by FastQC (version 0.11.5)