FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1973-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1973-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137674
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14250.12525556530253834No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA12270.10785163412365933No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11430.10046814816898338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2250.067.646241
GCGATCG350.003574012562.110422
CGATCGC350.00357900262.0885543
TTACGTG3050.047.516172
GTGATCG1307.403287E-944.5803269
TAATCGT700.00106079741.396019
TCGCCAT1458.65748E-735.02182145
CGTGTTG4150.034.904625
CTATACT2951.4551915E-1129.4644584
CAACCCG3600.028.212025145
AACGTAG1306.521752E-427.8565715
ACGTGTT5300.027.3333824
GTAGCAT16100.027.0104771
AGCGAAA4650.026.4973751
CTAGTAC1658.469774E-526.34063
GCGAAAG5250.026.2301751
GTAGCGG3057.185008E-1026.1269939
TACGTGT5550.026.1032963
GGTAGCG3151.0440999E-925.2975658
GTTGACA8550.024.5832211
Produced by FastQC (version 0.11.5)