FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1973-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1973-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATGAGTAAGCTGCTTCATATCTAGCGTGGTATTTAAGGGAAGATAGT17.6923076923076925No Hit
GTCCATTTCCAGGTGGCTTTGATAATACCCAGATTGCCGGACCAGCTCCT17.6923076923076925No Hit
ATTCAAAACAATTCAGTTTATGCACACCCCACACAGATCATACTTGGCAT17.6923076923076925No Hit
AATAAGAATAAAAATATACAAAAAAAAAAAAAAAATAATAAATACAAAAA17.6923076923076925No Hit
ACCTAAGGTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCATT17.6923076923076925No Hit
GAGAAAGGGTTAAGATGAGTGGCTTAACCATAATTTTAGTATTTCTTGAA17.6923076923076925No Hit
GTACAGACCATGTGAACACCTGCACTTTACAGCCGGTCCTCGACTGCTGT17.6923076923076925No Hit
ACTTAATTCTTATTAAGAATTCATGGGTCCTGACCTCTCTCCCCTTAATC17.6923076923076925No Hit
GCATGGCTGGGAGGGAGGCTCCAAAATCAGATTCTTTGAGTCAGTCTGGG17.6923076923076925No Hit
GTAGAAGTTCTGGTGGGAGATGCAGTAGTGGTGGTAGTTGTAGTAGTTGT17.6923076923076925No Hit
CTTAATAATGTTCTATGAACAATTACACGCCAACAAGCTGGATAATCCAA17.6923076923076925No Hit
ATGTATGGGTTCATTATAAAAGGAAGGTCCCATTTGAGAAATGATACTGA17.6923076923076925No Hit
CAGCTAGAATGCAAAATATTACCATTCAAGCAAAAATTTTAGAGGATGAG17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)