FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1972_lungMR_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1972_lungMR_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7571
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG140.18491612732796198No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA120.15849953770968167No Hit
GTCCAGGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGC110.14529124290054155No Hit
GTCCAGAGGGCACACGTGGGCTTGCCACCAAGCACCCGGCCCTGAACTCG100.1320829480914014No Hit
CTACTGTGACAGCCGCAACCTCCGGAAGGTCCCTGTCATCCCCCCACGCA90.11887465328226125No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC80.10566635847312111No Hit
GTTCATGGGCAAACCAAAATAGGAACACAGAAATACTTTGAGGTAAATGA80.10566635847312111No Hit
CCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTCTA80.10566635847312111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC100.0069530634145.04
GTTCTAT100.0069530634145.01
TCTATAC100.0069530634145.03
TGGACCA100.0069530634145.05
TTCTATA100.0069530634145.02
TTCAGAG100.0069530634145.01
TACCACT100.0069530634145.07
AAAAAAA1059.349606E-1017.952381140-144
Produced by FastQC (version 0.11.5)