FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1972-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1972-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452626
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG5630.12438525405080575No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT4940.10914088010852227No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC4830.10671061759598433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1601.0168151E-940.7444735
TACAGCC2109.094947E-1237.954537
ATAGGAC850.002751062934.0906453
TATGACA1707.8611265E-834.0906454
GATATAC900.003640232632.196723
GTAGAAA2558.913048E-1131.2601262
TAGTACC2401.5861588E-930.1911019
GCACCGT1204.073417E-430.1844256
AGCACCG1204.0760566E-430.181095
TATAATA3400.029.839212
TAAGGTA1703.0255178E-629.8293154
TCAGGCG2701.72804E-1029.5201879
TAGTGCA2252.8256181E-828.9834589
TAGTTCA3102.910383E-1128.0392045
CATAGCA2405.2817995E-827.1749952
GTATATC1100.00967734226.3573421
CTGTACT1658.453969E-526.3427724
CGGTCCA1409.961342E-425.903868145
GTTGACA5100.025.5821251
GTAGTTC3151.038643E-925.2974224
Produced by FastQC (version 0.11.5)