FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1972-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1972-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866697
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG13580.15668682365347983No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11930.13764902843785085No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA10230.11803433033689974No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9980.11514981591028929No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT9400.10845774244055305No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC8920.10291947474146097No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA8770.10118876608549471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1750.053.835821
ATCGAAT450.009620761548.356125145
CCACGAC450.009662610548.303033
TTACGTG2100.044.860592
GCTACTA2501.8189894E-1234.7801939
GTTGACA9750.034.191591
GTTATGT7700.032.94151
TACACCG1102.4473527E-432.9281775
AGTCTAT3000.031.404231
CAGTGCA6650.030.5124682
GTGATCG950.00474012830.5089429
CGCTCTA950.00474012830.5089429
TATACTG4750.030.5018925
CGTGTTG3100.030.3788995
GCATGTT14000.030.0134144
TAGCATG14250.030.0039252
GCAGTGC6550.029.8736651
CAAGCGG1000.00609264828.981823
TACGTGT3251.8189894E-1228.981823
ACATGGT11900.028.6115425
Produced by FastQC (version 0.11.5)