FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1972-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1972-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779452
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG54770.3077913874608587No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC41820.23501617351858886No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT34550.1941608989733918No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA29650.1666243315357762No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC28480.1600492735965904No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG28080.15780139054045852No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA27310.15347421565740466No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA26740.1502709823024167No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA26480.14880985831593097No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC25950.14583141326655621No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT24420.13723326057685176No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC24030.13504157459712315No Hit
CTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTTTA22830.12829792542872748No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA22520.12655581606022528No Hit
GTCTGTCTGTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCT22190.12470131253891648No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG21540.12104850257270215No Hit
GATACTTGGGTGAGGATTTTTATGTTTATGTTCATCACAGATACTTGGGA21510.12087991134349227No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT21380.1201493493502494No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT20930.11762048091210103No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA20760.11666513061324497No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT20390.11458583878632299No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA19040.10699923347187786No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT18830.10581909486740862No Hit
AAATTAATACAATGAAACAAAATTCACAGAATGCAGTAAAACAGGTTCTA18550.1042455767281163No Hit
GTTCATGGGCAAACCAAAATAGGAACACAGAAATACTTTGAGGTAAATGA18310.10289684689443718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT1100.065.866154
CGAATTA808.132702E-963.3961687
GTTACGT2700.061.7416041
CGCCGCG400.0060369554.40845145
TTACGTG3100.053.7643552
TATACTG5800.052.461375
GCTACTA4000.050.7169349
GTTATGT11700.045.841591
GATATAC5300.045.1120953
GGTTATA5850.044.602631
TACGTGT3850.043.2834743
CTACATC7550.042.224133
CAGTGCA10500.042.0987132
CATTCTA24150.042.0086942
AAGCGGC2550.039.777994
GGTGCGA1105.2902415E-639.5196888
ACGAATT1303.7203063E-739.0119326
ACGTGTT4300.038.7538034
GACTTAA8800.038.710521
GTGCGAA1701.8426363E-938.3573464
Produced by FastQC (version 0.11.5)