FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1972-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1972-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844607
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11520.13639479663322707No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC9320.11034717922063161No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8740.10348008008458372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGT4650.043.637791
TATACTG3750.040.5639085
GATATAC2302.7284841E-1134.6471943
GCTATAT950.00473648730.5136471
TCTACGG1050.007736828627.5977653
GTATTAT9000.026.5722981
GTTACGT1658.440837E-526.3526941
GTATAAG2259.1377115E-725.7670761
CGGAGTA1751.2625945E-424.8365174
GCACCGT2651.3876706E-724.600756
GACTTAA4550.023.8911761
TACCCAC6100.023.7592162
GTAATAT2151.8712894E-523.5948521
TGCGTTA2151.8764478E-523.586468
GCGTTAA2151.8773088E-523.5850629
TGTGTTG8050.023.395335
GTAGGCT2802.3619032E-723.2828526
GGATATA3152.878187E-823.0049552
CCGACTA2852.8000613E-722.8757384
TAAGGTA2852.8000613E-722.8757384
Produced by FastQC (version 0.11.5)