FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1972-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1972-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949306
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.042.6449621
GCGAAAG3950.036.695442
TGATCCG1854.2073225E-935.248079
CGAAAGC4450.034.188343
TTCCGAC2101.45082595E-831.0469594
GTGATCG1204.0728835E-430.189329
GTACCAC2003.2720163E-728.9817479
GTCCTAT10350.028.7183951
ACCTGCG1658.454243E-526.3470427
ACTATAC5050.025.8225943
TCCGACT2559.577343E-825.5626895
GCGCGGT1450.001221847125.01594145
CTATACG1500.001501087224.1476354
GTATATA8500.023.8811781
CCTATAC4303.6379788E-1223.5873053
TATACTG7800.023.2139825
CGACTGG3152.8838258E-823.0013877
GTCTATA3706.6520442E-921.5529961
CTATGAT10650.021.0866664
GGGTTGA9550.020.480166
Produced by FastQC (version 0.11.5)