FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1971-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1971-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720566
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC7760.10769311902032569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTA350.00355792462.17886145
ACCGTAC350.003568718262.1313321
AGTCGCG400.00606463154.3422629
GTGATCG901.3285571E-648.3042349
GTTACGT951.9225827E-645.7809831
CCGTACA700.001057769341.418012
TATACTG3900.037.1571045
AGCGAAA3050.035.6491241
GGACCGT850.00275052134.0947386
TTGAACG900.003635800732.20734
ACCGTGT4950.030.7390568
TTACGTG1703.0161555E-629.8453312
CCGTGTT4900.029.574029
GTATTAT8150.029.3503861
GCGAAAG3650.027.801132
GCACCGT5050.027.258916
AGCACCG5450.026.5894835
CGAAAGC3851.6370905E-1124.469183
ACAGCGC1500.00149911624.1521178
GTAGCCG1851.8374587E-423.4993559
Produced by FastQC (version 0.11.5)