FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1970_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1970_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832137
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC10290.12365752273964503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3750.042.519681
GCGAAAG4100.038.8712352
TACCACG750.001490423838.6286777
ACTGATC7200.036.2143868
GCGTGCG1001.399314E-436.2143869
AAGGCGT1001.3998094E-436.2122086
GGATAGC5900.035.6005825
GGTACTG6000.033.8000955
CGAAAGC4950.033.6557963
GTACCAC2009.027644E-932.594913
GCAGGTA6200.031.5472532
GTACTGA6200.031.5396616
CGCTAAA1000.006083403228.9906961
TGTATCG1000.00610311228.9715085
ATCGTGC1504.427403E-528.9715088
CAATTCG4300.028.6346289
GTATAGG3301.8189894E-1228.551441
CACGAAG1306.5193826E-427.8572187
CAGGTAC7400.027.407133
AGGTACT7750.027.104014
Produced by FastQC (version 0.11.5)