FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1970-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1970-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474493
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11150.23498766051343223No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT6900.14541837287378317No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA5170.10895840402282014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT353.4371915E-582.848461
GAATCGT502.0154529E-457.963319
ACCGTAC450.00966014448.302768
TATACTG2350.040.0810175
CGCGAAC750.001478747338.687122145
GGAATCG750.001487214438.6422128
TCTATAC5050.037.3070643
AGCGAAA2002.237357E-1036.2461971
GCGAAAG2052.910383E-1035.3621441
GTAATAT1905.4187694E-934.33851
ATGTGCG850.002749049834.0960665
GTTCTAT5750.032.779171
TTACGTG900.003631933832.212042
GCACCTA1153.1713533E-431.50186
TATACCA6600.030.7381215
GTTGACA4900.029.5887341
TCGATTG200.006076090529.00002340-44
GTGTTAC2054.0352825E-728.2897171
CTATACC7050.027.7483944
ATACTGT4500.027.3715656
Produced by FastQC (version 0.11.5)