FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1970-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1970-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843416
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT50470.1774977702875696No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG49360.17359401508607958No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG42730.1502769907744769No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC41010.14422792866045628No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT28550.10040739729958613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG19200.043.7675825
GACAGTA23100.042.9669957
TATAGCG1053.840414E-641.4068762
AGCGAAA13650.040.351561
ACAGTAT23900.040.013838
GTTACGT4400.037.883891
AAGGTCG8600.037.0670749
GCGAAAG15500.036.4647642
GCAGGTA22800.035.9132042
AGGTACT23650.035.8417134
CAGTATG26550.035.2013449
CAGGTAC23300.035.1363453
TTGGACA28600.033.9447674
ATCCAAT38400.033.771337
GTGATCG4500.032.199689
GTACTGA26550.031.6528076
ACTGATC27750.031.5904988
TATACTG23100.030.733355
TTTGGAC33250.030.722853
CGAAAGC19050.029.6642683
Produced by FastQC (version 0.11.5)