FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1969_lungLR_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1969_lungLR_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894850
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12130.13555344471140413No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9550.10672179694920937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT907.490093E-540.2702061
ATCGAAT850.002735332634.13416145
GTACTAG1102.438632E-432.948351
CAATTCG1102.442641E-432.9391379
GTATAGT1153.1653195E-431.5158121
TATACTG3100.030.3822085
CTTCGAC1000.00609300928.9815713
TTACGTG1306.495907E-427.8746952
CTACATC3700.027.4150033
ATATAAC2756.7884685E-926.3542582
TAACCTA2507.881499E-826.0819575
CAACCCG3357.4578566E-1125.982718145
TAGTCGG1751.2595738E-424.8455168
CAGCAAC6400.023.7766067
GTAAATT3402.5211193E-923.4409774
GTGCGAA2552.721532E-622.7306444
TACACCG3203.4095137E-822.6405875
ACCGTGC2603.2165626E-622.2972588
TGTAATA3604.8657967E-922.1448962
GACGATG2303.1298252E-522.075897145
Produced by FastQC (version 0.11.5)