FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1969-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1969-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20125
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC300.14906832298136646No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG210.10434782608695652No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG210.10434782608695652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAAC100.007039489145.0145
GTTTAGG100.007039489145.01
CTCCTTC259.252197E-487.09
GCTCCTT259.252197E-487.08
ACTGAAC502.224766E-672.58
ATGAAAG300.00190809772.51
CTACTGA400.005965104354.3756
TGCTCCT400.005965104354.3757
AACAAGG450.0095029948.3333327
TACTGAA450.0095029948.3333327
CAGTAAA400.00786779918.1251
Produced by FastQC (version 0.11.5)