FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1969-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1969-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344585
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14810.11014550958102313No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14690.10925304090109587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG2807.2759576E-1231.0486039
TATACTG7700.031.0474475
TGTAGCG1100.00969864726.3491742
GTTGACA11150.025.9995421
CTACATC6000.022.9431763
GGACTGT8150.022.2212726
CTACTGA16000.022.1851626
GTATATA8050.021.607071
CCGACTA2353.7304184E-521.5806834
ACATGGT13050.021.0948185
TACTGAA17150.020.6998397
TATACGT2104.3355548E-420.6998394
GTTACGT2155.06975E-420.2252251
GTCCTAT19100.019.7310661
ACTGAAC20200.019.7263348
ATGTTGT26250.019.3176946
GTAATAT8300.019.2099041
TAGGACT10200.019.1777944
TTAGAGT8500.018.751624
AGCACCG8500.018.7502235
Produced by FastQC (version 0.11.5)