FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1969-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1969-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655228
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCG450.00966614848.2972375
GCGAAAG2302.7284841E-1134.6612551
AGCGAAA2901.19362085E-824.9908731
CGAAAGC3201.2460077E-924.9108732
ACTGCGT1550.001814086723.3732018
TATACTG3102.4338078E-823.369635
TATAGGG2603.2136522E-622.2978442
ATAGGAC3809.105861E-920.971173
GTATTAG2104.3225763E-420.7067241
GTCCTAT6650.020.7067241
CTCTATA3157.3047886E-720.7051412
ATATAGG3651.3635872E-719.8557621
TCTACAC3351.3224253E-619.4630683
GTACAGG3451.746228E-618.9061371
GTATAGT2701.08147084E-418.7894331
GTGTTAG3502.0039133E-618.636051
TAGTACC2801.43163E-418.1142319
GTTATAC2000.00794738918.1114643
GTATTAA11100.017.6286961
GTAATAT3302.5176303E-517.5693421
Produced by FastQC (version 0.11.5)