FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1969-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1969-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19822
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG280.14125718898193926No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC270.13621228937544144No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT240.12107759055594793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGA100.0070752366144.754
GCTACTA100.0070752366144.759
CTACTGA301.5743008E-596.56
ACTGAAC301.5743008E-596.58
TTCTACT300.001920847572.3754
TACTGAA406.5591725E-572.3757
TATATAT350.003539218362.0357133
TGCAGCT350.003539218362.0357135
TAAAATT400.0060048654.281254
TCTACTG400.0060048654.281255
ACCTGAG450.00956624348.257
CTGAACA700.001040522641.3571439
AATTGGG400.0079457818.093755
ATACAAT500.00139748417.3780-84
AATACAA707.818855E-414.47580-84
Produced by FastQC (version 0.11.5)