FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1969-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1969-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235475
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT2808.26185E-925.8745147
TAGACCG1851.8413356E-423.4945
AGACCGT2953.9100632E-722.0984956
ATCGATA4006.9667294E-1021.733718
GACCGTG2455.1432362E-520.6996127
TACACGT2104.33916E-420.6970965
GTATTAG3901.22727215E-820.4383741
TCTATAC11350.020.4268783
GCGAAAG6100.020.1946961
TAGAGCG1800.004328383620.1221755
CGAAAGC5453.6379788E-1219.942422
ACCGTGC3001.113038E-519.3188558
GTATAGG2659.388324E-519.1412561
GAGTACG1900.005912803619.0654329
GCGTCCA1900.005912803619.0654329
AGCGAAA6151.8189894E-1218.8522471
GATGCGC6750.018.2455848
GTCTACA4455.574111E-817.9122831
GTCCTAT9350.017.8252031
ATAACGC2450.001222608717.7432443
Produced by FastQC (version 0.11.5)