FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1968_lungLR_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1968_lungLR_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843668
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14860.17613563629294937No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10940.12967186144312692No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10620.1258789002308965No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8710.1032396629953963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1351.0273652E-842.955241
TAGTCGG1601.0168151E-940.7521488
TTACGTG1553.4802724E-837.4059682
TATACTG5250.037.2546845
CTACATC4450.034.1931273
GTTGACA12250.033.728631
ACATGGT12550.033.4780965
TACGTGT1751.01415935E-733.1231353
GTATATC2008.962161E-932.619141
ATGCGCC900.003640613332.199237
GGTCGAT1153.174944E-431.4992457
CTGATCG1153.174944E-431.4992459
TTGACAT13650.030.2638952
TGACATG13800.029.9278343
GACTTAA3900.029.7382431
AGCGAAA2502.4501787E-928.9947871
GACATGG14450.028.5799054
AGTCTAT2303.4924597E-828.3644661
CGTGTTG2054.0673876E-728.2691425
ATACTGT6450.028.077396
Produced by FastQC (version 0.11.5)