FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1968-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1968-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743578
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG400.0060535154.367651
GCGAAAG3350.041.1083072
AGCGAAA4200.032.7931861
TATAGTA1352.1454189E-532.2135242
GTATAGC1902.0778316E-730.522191
GTCTAGC1952.6088674E-729.739571
CGAAAGC4650.029.6096783
GTATTAG1504.401254E-528.996081
TCTATAC3057.1486284E-1026.1352043
TACTACA2051.29238215E-524.7494162
TATACTG1500.00150037424.148765
GACGTCT1600.002169526622.665401145
GTATATA2603.206078E-622.3046781
CTATACC4307.2759576E-1121.9067864
ATAGTAC1700.00310052921.3134673
CTACTAC1750.003660720320.7114871
GAGTATA3901.2125383E-820.456978145
GTCTATA2556.96163E-519.899271
TAAGGCA5950.019.4852894
TAGATTG5356.548362E-1118.9579055
Produced by FastQC (version 0.11.5)