FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1968-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1968-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438214
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG25830.17959775109962772No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC20120.1398957317895668No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT14920.10373977725150778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1650.065.8780751
TTACGTG2400.045.2895972
CGAATTA1201.9826439E-742.2688187
GCTACTA2750.036.889159
CGTGTTG3300.032.9310075
ACGAATT1652.4000728E-630.7366776
TAGTCGG3100.030.3867998
CTACTGA21000.029.6703056
TACGTGT3700.029.3729463
TAGCATG22250.029.3110222
TATACTG5450.029.2451535
AGTCCGC2003.2475873E-729.008572145
TACTGAA21700.028.7171967
ACGTGTT4300.028.6453134
GTTATGT8450.028.3002831
GTTGACA18200.028.2696571
AATCACG1804.7203703E-628.1801932
GCATGTT22800.027.9653914
AGCCGGC5000.027.5351167
ACATGGT18200.027.4666355
Produced by FastQC (version 0.11.5)