FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1968-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1968-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699253
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10960.15673869114612307No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC8390.11998518418941356No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7910.1131207159640359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2650.054.6824875
GTGATCG604.9594464E-448.3028649
GATATAC1701.8389983E-938.3581543
GTTACGT951.03065715E-438.144771
GACTTAA2103.783498E-1034.5119361
AGCCGGC1751.0140502E-733.1219647
TGCGGAT1102.4280263E-432.971565145
GGTTCGA1102.444431E-432.9337738
GTTGACA6650.030.5158181
AGCGAAA2401.5861588E-930.1979431
TCGGTTC1204.071818E-430.189298
TAGCATG9950.029.1294422
GTCCTAC3001.8189894E-1128.9900251
ACAGTCG1000.006091957428.9817184
GTCGGTT1255.171315E-428.9817167
GCATGTT10500.027.6016374
AACCGCG1050.001293665127.6016357
GTTCGAT1050.007730636327.6016359
GCCGGCC1855.8414007E-627.415148
CTACATC2654.5838533E-927.3412443
Produced by FastQC (version 0.11.5)