FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1968-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1968-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083488
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16730.15440872441596032No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12160.11223013083670516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG950.0047439130.504657
TATACTG4500.028.97545
GTTACGT1804.7078047E-628.188761
GTAGGCT3403.6379788E-1227.6983556
CATTACC7200.027.1682033
CTACATC4850.026.888123
CCGACTA2601.15436706E-725.0748674
ATTACCT8500.024.7143144
TACTGAA11750.024.6633347
TACGTGT2101.563795E-524.1495173
GTTGACA8450.024.0188271
TACTATA3052.0454536E-823.7591112
ACCTCTA9100.022.291867
AGGTCGG5900.022.1029458
TTACGTG2303.153967E-522.0546512
GATATCG2003.119169E-421.733569
GTATTAT10150.021.4242521
CTATACT2755.249756E-621.0730194
CTACTGA12350.020.5300776
TAGCATG13450.020.4734652
Produced by FastQC (version 0.11.5)