FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1968-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1968-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821668
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG8430.10259618239970403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2600.044.5776375
GTTACGT1105.273916E-639.533681
GATATAC2052.9467628E-1035.3423963
TTACGTG1352.1487858E-532.2067382
CGGAGTA1153.1762046E-431.4969924
TAGTCGG1652.3992561E-630.73538
CTACTGA11650.029.2241826
GTATAAG2054.048179E-728.2842621
GTAGGCT3351.8189894E-1228.1105256
ATAAGAC1306.5071456E-427.866123
TACTGAA11950.026.6736037
TACTTAG3005.9117156E-1026.562467
TAGGCTC3850.026.3429367
GGATATA3108.62201E-1025.7134422
ATTACCT5800.024.9803714
AGCGAAA3902.0008883E-1124.1594731
CTTATAT2451.91634E-623.666421
ACTGAAC13500.023.6125168
AGCCGGC3702.6193447E-1023.495057
CATTACC5250.023.4606193
Produced by FastQC (version 0.11.5)