FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1967_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878266
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCT502.018253E-457.952564
GTCTACG805.8875594E-754.355321
TCTACGC850.00275128334.093632
GCGAAAG3150.032.1995432
AGCGAAA3251.8189894E-1228.9895061
CGTAGAC1000.006098384528.976283
ATTAACG1100.00970959126.3420733
TACGGCA1100.00970959126.3420737
CTTCGCC1100.0097122926.3405749
CTACTGA11700.026.0028746
CCTTCGC1400.001004477525.8702059
GTAATAC2601.1535121E-725.0756283
TACTGAA12150.025.0412317
CGAAAGC4455.456968E-1222.7903353
TAGGGTG3854.3291948E-1022.5776355
ACTGAAC15250.021.3747278
CATTCTA11050.020.9806982
TCAGGCG2155.085371E-420.2148599
CCATACC3601.1858356E-720.1224173
GTATATA5707.2759576E-1219.0720421
Produced by FastQC (version 0.11.5)