FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1967_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286645
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1351.0244548E-842.949255
AGCGAAA1303.6902384E-739.032821
CTATACT1051.8538962E-434.5127944
CGAAAGC1551.463819E-632.7314873
GCGAAAG1652.3845114E-630.747762
CTAGAGT950.00472910130.5165734
GCTTGTA1050.007704848427.6150557
CTAGGGC1855.8115547E-627.423683
ATACTGG1658.416244E-526.3552256
TTATACT1409.99573E-425.8845944
ATATAAT1751.2553867E-424.8492092
TCTGTAG2101.553875E-524.1589553
TAGGGCT1851.830183E-423.5060124
GTTATGG1600.00217321222.6529731
CCGTGTT1952.6140703E-422.3005739
TAGGGTC2353.7064056E-521.5888545
CTCTGTA2704.43884E-621.4746252
GTAGTTC1700.00309319121.3167254
TCTAGAC2053.6655195E-421.212743
ATAGCAC1750.003659209520.7076743
Produced by FastQC (version 0.11.5)