FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1967_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585629
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGGACTGTGCAGTTTAAGGCAGTTAACTCTACCTTGAAACCCAAAG9290.15863285458882673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1000.072.4730761
AGCGAAA1200.066.433651
TCGCGGC400.006029896454.419933145
AAGGTCG400.006066733554.336229
TATAGGC1101.6825652E-952.6941762
CCGGTTG450.0096057548.373272145
CGAAAGC1903.6379788E-1241.9437563
GCGAAAG2005.456968E-1239.849972
CATAGCG850.002751815834.0904055
CGTTTAT2154.8748916E-1033.7084051
CTCGTCT900.00361236532.248848145
TGTACCG1352.1524174E-532.196495
GTATAAC1204.0648444E-430.1971131
AATAGAC1952.6224552E-729.7198414
GAACCCC2202.2784661E-829.6354066
AACCCCG2252.834895E-828.9768417
ACTGTGC10100.028.6923968
CTTATCG1804.721722E-628.1743379
TAGTAGT1306.5100455E-427.8623494
AAGACCG1050.007735976527.5969925
Produced by FastQC (version 0.11.5)