FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1967-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584997
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGGGAGGATGACCAAGAAGCATGGGGCTGCGGTCTTTGGTATCAA8660.14803494718776336No Hit
TCCCTATCCTATGGGGAATTAATGGAAGATCCAGAACTAGAGCCTAGGTT8560.14632553671215406No Hit
TCTATGGGCAGTGTATTTTTTGCTACCCCAAGCTGCCTGCAGGCTTTCTG7260.12410320052923349No Hit
CAATATACAGATTACCTTTTTTTAAACTGGAGTTACCCAACAAGTGGACC7160.12239379005362419No Hit
TCACTAGACAGTGGCTATGGAGAAAAGTAGATGGAATCAAAAAATACTGA7140.12205190795850235No Hit
TGGTAGAGCAGGTGTTGAGGCACAAGGACACCAGCCAAGGGAGCAAACGA7070.12085532062557586No Hit
GTGCAGTGTGGTCAGTGACGCAGCACTCCGGGGAGAGATGGAGACGCAGA6490.11094073986704205No Hit
GTATAGTGGGGTCGGTGATAGTCTGCGTTAGATAGGGAATGATGTTTAGG6270.10718003682070165No Hit
GGATAATGGGTTGTGCCAGGTAACCAAAGCAGAATCCGTGATGGAAAATC6000.10256462853655658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2050.091.910661
GCGAAAG2250.080.5062262
CGCATAC350.003571999362.115441
GCCGGTG651.0981641E-555.7255448
GTGCGAC553.2072206E-452.762745145
CGAAAGC3700.050.9060363
TCCGCCG450.00961156548.365852145
GCCTAAG3150.046.011441
TATCCTA2900.044.964755
GAGCGAA657.3500205E-444.5957031
GTATATG1053.832987E-641.4102941
GTTATAT1601.0131771E-940.7632561
ATCCTAT3550.038.772426
AGTATAC951.0310407E-438.1410641
TATAGGT800.0020437736.22164
CCGGTGC1051.8603909E-434.4997189
GTTGTGC3500.033.116898
CTACAGC1751.0149415E-733.116894
CCTAAGG6350.033.0899622
ATAGGTT1102.4461432E-432.928735
Produced by FastQC (version 0.11.5)