FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1967-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933897
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGACTGCTCAGGGTATAGCCCAAGGAACGGGGCTAAAGATAGAAG17930.1919911938896902No Hit
GTATATACTCAATAGGTAAGCTGAATGCTTACCTGCTTTATAAAGTCTGT12600.13491851885165065No Hit
ACTTATTACCGTCTTCAGAAAATCCAAGATATGGAAGGTGGAAGAAAGAA10980.11757185214215271No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA203.857684E-4108.80869145
AGCGAAA2800.0103.532861
CGAAAGC3750.079.220163
GCGAAAG3800.078.190362
CGTTATA451.1960187E-464.420441
TTACCGT3350.062.7216
CCGTATA350.003558812762.176395145
ACCGTCT4100.054.7849048
CCGTCTT4000.054.3430949
ATTACCG3950.053.1937645
TATTACC4100.053.017654
GAGCGAA1004.7166395E-850.7311021
TACCGTC4500.046.6948057
TCTACAC1704.0017767E-1142.6220363
TATAGGT1105.285183E-639.5222474
CCCTATA3800.038.141642
GCATCGA800.002040808336.234562
GTATATA8250.034.2599641
CCTATAG4750.033.5592423
AGGTTCG900.003638550432.2033128
Produced by FastQC (version 0.11.5)