FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1967-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966164
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14610.15121656364757952No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11120.11509433181116249No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG400.0060672254.337812
TCGACAA803.7187798E-545.344982145
ATCGATG855.318814E-542.677628145
TATACGG700.001061808641.3873635
ATAGCGG750.001489873438.632213
AGACGGT2351.2532837E-930.873177145
GTTACGT1453.499165E-529.988811
TACGGGG1050.00774388627.5930047
GCGTTAA1050.00774388627.5930049
GTTGACA10800.027.512881
TATACTG4550.027.0609685
GCTATAT2456.461232E-826.6227191
GTAGGAC4450.024.4164243
GTTATAC2701.663011E-724.1451283
TTGACAT12100.023.950552
TTATACT4900.023.6511484
ACATGGT11950.023.6375525
GTATAAG3702.6193447E-1023.5047421
GGTCCTA14900.023.3336819
TGACATG12900.023.02213
Produced by FastQC (version 0.11.5)