FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1967-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1967-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864369
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12800.148084903553922No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA10290.11904637949764511No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC9800.11337750428347153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1950.052.0250131
TTACGTG2000.050.7097052
GCGAAAG2950.041.746492
AGCGAAA3000.041.0626031
TATCCCG750.001491194738.6247835
GTAGCAT14500.035.4820861
CGAAAGC3650.033.7285963
TACGTGT3100.032.7045753
CGTGTTG3350.032.427525
GTTGACA7650.030.3114971
ACGTGTT3400.029.8206044
GCATGTT13800.029.3884224
TAGCATG14000.028.9769742
TATACTG5000.028.9685885
ACATGGT8500.028.1165685
GTACTAC1050.007726882627.6051121
GTATAAG2105.0071503E-727.6051121
CATGTTG12550.026.544925
AGCATGT15650.025.9128913
CTACTAT2001.0694259E-525.36221
Produced by FastQC (version 0.11.5)