FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1966_lungUL_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966_lungUL_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTAATACAATGAAACAAAATTCACAGAATGCAGTAAAACAGGTTCTA40.3215434083601286No Hit
AGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACGTCTCAAATA40.3215434083601286No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC30.2411575562700965No Hit
ACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAGTCCTTC30.2411575562700965No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA30.2411575562700965No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT30.2411575562700965No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC30.2411575562700965No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA30.2411575562700965No Hit
GTATTAATTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT30.2411575562700965No Hit
CATATAACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTC30.2411575562700965No Hit
GTTGAGATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT30.2411575562700965No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT30.2411575562700965No Hit
GTCTGTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCTTTAA30.2411575562700965No Hit
GTATCAAGTTAGGTTGCTTATTTGAGATGTTTCTTGTTTCTTGAGTTAGG30.2411575562700965No Hit
TTCATAAACACAGAGACTAAACAACATGCTACTGAACAACCAATTGGGTT30.2411575562700965No Hit
CTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTTTA30.2411575562700965No Hit
GTCCAGGTGACACACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGC30.2411575562700965No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT30.2411575562700965No Hit
GAGCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAGGACCC20.1607717041800643No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC20.1607717041800643No Hit
GATACTTGGGTGAGGATTTTTATGTTTATGTTCATCACAGATACTTGGGA20.1607717041800643No Hit
CTTTAGTGAAGCTTTCTCTAACAAGCCTATCTAAAATATCTCCCCATCCC20.1607717041800643No Hit
CTTCTAGGAACAGGATGATTTTCTCACGCTGTAAGTGGGGAGACCAAGCA20.1607717041800643No Hit
GTGTGATATCATAGGGGAGACTCAGGCCAGACAACCCCATAAGCCAGCTC20.1607717041800643No Hit
TTTTAGAACCTGTTTTACTGCATTCTGTGAGTTTTGTTTCATTGTATTAT20.1607717041800643No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA20.1607717041800643No Hit
GATCATACCTCTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTG20.1607717041800643No Hit
GACTAAACAACATGCTACTGAACAACCAATTGGGTTCATTGGCAAACCAA20.1607717041800643No Hit
GCTCTTAAGAGTGTACTATGAACAATTACACCCCAACAAGCTGGATAATC20.1607717041800643No Hit
GAATATACTCCTCTCAAGTGCCCCTAGAACGTTCTCCACCTTAGGTCATA20.1607717041800643No Hit
CTTCCAGATGGACCGCTTAGGACCCTATGGCTCATGTCTCCTAACATCCC20.1607717041800643No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20.1607717041800643No Hit
AAATAGAGATCACTTAGAATAGTACCTATGAGAGTAAGCCCTCAAAAAAT20.1607717041800643No Hit
GTCCAGGACCCCATGGTTTCACCTGTGAATTCTACGAATCATCCACAGAA20.1607717041800643No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20.1607717041800643No Hit
CAAATAAGCAACCTAACTTGATACCTCAAGGAAACAGAAAAGAACAAATA20.1607717041800643No Hit
ACATATGACCTAAGGTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATA20.1607717041800643No Hit
AATTAATGCAGTACAATTATCCAACATTTATAAATATTTACTGCCCCAGC20.1607717041800643No Hit
TATCAATGGTTTCAGGGAACATCTCTCTCATTTCTGATGATGAGTCCTCT20.1607717041800643No Hit
GGATATGGTCATTGGAGAAGAATTTATTGTGTTTATTTACAGAGGAAAAA20.1607717041800643No Hit
GTACCCTTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA20.1607717041800643No Hit
AAATACATATGTCTTTAAAAATCTTATCCTGAACATTTGGTGGTTGCGCA20.1607717041800643No Hit
TTCTCCTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATC20.1607717041800643No Hit
TTCTTATGAGCCTTTGCATGTCAGTGGTTTCAGGGAACATCTCTTTCATT20.1607717041800643No Hit
GTCTACTAATGACCAGTAGAGAGATTAAATAAGAAATCAAAAACTTCCAA20.1607717041800643No Hit
TAGTTTCTCCATGCTGGGTGGGTAAATATTTATAAATGTTGCATCATTTT20.1607717041800643No Hit
TTGCTGATCTGTTATTCTTGTTGGTGTTTTTAAAAATATATATATCTTTC20.1607717041800643No Hit
GGGTGGAGGTGGCAGGTGCCCAGGCATGTCCCAGCCCCTGGGTCAGAGGG20.1607717041800643No Hit
ATGATATTGGTGCTGATTGCTTCTCTTAGGTTTGTTCATATATGCTCTAC20.1607717041800643No Hit
CTTACACAGTTTGGCGACAGTGGCCATTGCTGCTATAAACATTGGGGTAC20.1607717041800643No Hit
ATGATACATTTCCAGAAACATACAACCTACTAAGATTGAATTCTAAAGAA20.1607717041800643No Hit
GAATCTCTTCTATTTAAATTTAAAAATAGTTTTTAGAATTGAGTGAAATG20.1607717041800643No Hit
GAATTTATTGTGTTTATTTACAGAGGAAAAAAAAGAAAACCAGACTTAGA20.1607717041800643No Hit
CCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGACCTCTGGAGT20.1607717041800643No Hit
GTATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACT20.1607717041800643No Hit
ATCCTGGCAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA20.1607717041800643No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC20.1607717041800643No Hit
GTTTATGTTCATCACAGATACTTGGGAGGTCTCGATAAGGGCCGTGCTGG20.1607717041800643No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG20.1607717041800643No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA20.1607717041800643No Hit
TTTATGGTCCTGGAATTGAGTCCTGCATGAGGTCCCTGGTCAGCACGGGG20.1607717041800643No Hit
GAGTAAGCCCTCAAAAAATGTTGTAAAAAATCTTATTCTGGGGGTACCTG20.1607717041800643No Hit
ACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAGCAATCAGCAC20.1607717041800643No Hit
ATTGCATCCCTGAAATACATCTTACTGGATCATACCTCTTCCCGTATTGT20.1607717041800643No Hit
GCATATCAATGGTTTCAGGGAACATCTCTCTCATTTCTGATGATGAGTCC20.1607717041800643No Hit
GAGGCTGATGTTTCCTTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG20.1607717041800643No Hit
CTACAAATCATCCACAGAAAAAAATGAATACCAATCCTTCTCCAACTCTG20.1607717041800643No Hit
GCTTCTTTGAGATGTTTCTTATTTCTTGAGTTAGGCCTGTATCACTATAT20.1607717041800643No Hit
ATCTCGCTGAGAATCTGTCAGCTTTGTTCTTTTCAAAGAACCAGCTCTTT20.1607717041800643No Hit
TTCTACTCCATTGGACTGAAAATAAGAAGATCATCTGTTAGTTCTGCAGC20.1607717041800643No Hit
CCCCAGCTCTGTGTCAAATAAATAAATGGCATCTTTAAAAAAATAAAGTG20.1607717041800643No Hit
GAAAACAAGAAGATCATCTGTTAGTTCTGCAGCCATGAACAAAGCACACC20.1607717041800643No Hit
TCCAAAAGCAGCAAAAGGAATATACTCCTCTCAAGTGCCCCTAGAACGTT20.1607717041800643No Hit
GGTCTGTTAGGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGG20.1607717041800643No Hit
CCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTCTA20.1607717041800643No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG20.1607717041800643No Hit
GATTCGTAGAATTCACAGGTGAAACCATGGGGTCCTGGACTTTTGCTTGT20.1607717041800643No Hit
ACTCATAAGAATGTACTATGAACAATTACACCCCAACAAGATGAAGCTGG20.1607717041800643No Hit
GTACTATGAACAATTACACCCCAACAAGCTGGATAATCCAAAAGAAAAGG20.1607717041800643No Hit
GAAATACATCTTACTGGATCATACCTCTTCCTGTATTGTAGAATTTGGTT20.1607717041800643No Hit
GTCTGTTTCTTTAGAATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG20.1607717041800643No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA20.1607717041800643No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.1607717041800643No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT20.1607717041800643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATCA100.006238093145.08
TGTTCAG100.006238093145.05
GTTCATC100.006238093145.07
GTTCAGA100.006238093145.06
TCTGTTC100.006238093145.03
TCATCAC100.006238093145.09
TTTATGT100.006238093145.02
TTATGTT100.006238093145.03
Produced by FastQC (version 0.11.5)