FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n02_1966_lungLL_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966_lungLL_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19574
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC270.13793808112802697No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG270.13793808112802697No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT240.12261162766935732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA100.007038152145.01
TAGAGGT100.007038152145.04
GGACTGT100.007038152145.06
TTCTTAC100.007038152145.01
TGACATG100.007038152145.03
GACTGTG100.007038152145.07
AGTAACA203.8089915E-4108.752
TAGGACT259.2487E-487.04
CAGGGCA350.003514436562.1428574
TGAGAAG400.00596287654.375145
AGGACTG604.8262812E-448.3333325
Produced by FastQC (version 0.11.5)