FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1966-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562613
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG951.0339667E-438.12247
AGCGAAA2401.8189894E-1236.251761
TAGTACG800.002042500536.2259454
GCGAAAG2451.8189894E-1235.48982
GTACGTC850.002753503634.0859076
CGAAAGC3001.8189894E-1128.9833373
TAGTAGT1050.00773062227.6007214
TATATAG1958.784671E-626.0106872
GTACTAC1902.190785E-422.8958471
TATAGTA2003.1127664E-421.7375012
CTATATA1700.003090255721.3245621
TATAAGG2505.9949838E-520.2883383
ACTGTGC5901.2732926E-1118.4150588
GTATTAA6301.8189894E-1218.4135911
TAGTACC3151.6949196E-518.3955699
CTTGTAC2000.0079381318.1145863
TATACAG4506.326809E-817.7104635
GTTATGG2050.00911901617.6837841
GTTTAGG2050.00915181217.6727663
TAGGACC3854.987025E-616.9368084
Produced by FastQC (version 0.11.5)