FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1966-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823821
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11760.14274945649601065No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG11080.1344952362224318No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC9770.11859372363656669No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA8370.10159974072037492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG1151.4165562E-744.113038
ACCTATA750.001486111538.6514172
CTATAGA1504.405964E-528.9920851
ATACCTA1050.007731687727.6014465
AGTGTAC1606.85355E-527.1718243
CCTATAC1358.1087626E-426.8363693
CCGACTA1100.00969783626.3484334
TCTATAC9350.026.3484333
TATACTG2207.5250136E-726.3468325
CATTACC6950.026.0640983
GTTCTAT9500.025.1773381
TATACCA10400.025.0801585
GTATATC1751.2577897E-424.8503591
CCGTAGA2351.3331246E-624.6741161
ATTACCT7050.024.666624
CTATACT1500.001499075724.152734
TTCTATA10900.023.9355092
TAGTACC3052.0434527E-823.7596729
GCTATAT1550.001811090723.3807161
CTATTAG1550.001811090723.3807161
Produced by FastQC (version 0.11.5)