FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n02_1966-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n02_1966-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801167
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8590.1072185948747265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTT752.5637966E-548.289637
GTGATCG1157.1792256E-637.7966089
TATACTG4350.034.9639855
GTAATAT3350.034.621511
AGCGAAA6000.033.82811
GCGAAAG6400.031.6979922
CGTTGGT950.00471298530.5445145
AGTGCGT950.00474479830.5025259
CGAAAGC6800.029.8240873
GGGATAC3950.027.548046145
TAGGCCG1358.130038E-426.824224
TACCATG4400.026.33987
CTATATC3801.4551915E-1124.7771114
TAATATA4750.024.405072
CCGTAGA3301.7753337E-924.1629261
GAACCGA3652.2191671E-1023.8125766
ACTGTGC8250.022.8292528
TACTGTG10850.022.6983537
TTATACC4256.184564E-1122.1536524
AATATAC5900.022.0972653
Produced by FastQC (version 0.11.5)